PHOSPHOTUNGSTIC ACID-HEMATOXYLIN. REACTIVITY IN VITRO

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The Reactivity and Staining of Tissue Proteins with Phosphotungstic Acid

After aldehyde-fixation, treatment with phosphotungstic acid (PTA) in aqueous acidic medium was shown to produce an intense electron-opaque stain with minimal distortion of organelles. Mitochondrial matrix, cisternae of the endoplasmic reticulum, and the Z-band of muscle were densely stained, whereas membranes stood out in negative contrast. Staining of glycogen or lipid was not apparent. Under...

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The Reactivity and Staining of Tissue Proteins with Phosphotungstic Acid Lloyd

After aldehyde-fixation, treatment with phosphotungstic acid (PTA) in aqueous acidic medium was shown to produce an intense electron-opaque stain with minimal distortion of organelles. Mitochondrial matrix, cisternae of the endoplasmic reticulum, and the Z-band of muscle were densely stained, whereas membranes stood out in negative contrast. Staining of glycogen or lipid was not apparent. Under...

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Photocatalytic Decolorization of Methyl Orange Solution with Phosphotungstic Acid

Photocatalytic decolorization of Methyl Orange (MO) solution has been studied using phosphotungstic acid (HPA) as photocatalyst. The decolorization reaction of MO solution is photocatalysis. The paper reveals that the optimal loading of photocatalyst for decolorization of MO solution (10 mg/L) is 0.6 g/L. The results show that the photocatalytic decolorization reaction of MO with HPA in a h...

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Interference of the chemotherapeutic agent etoposide with the direct phosphotungstic acid method for uric acid.

A 62-year-old woman receiving chemotherapy with etoposide showed discrepant uric acid values as measured by a direct phosphotungstic acid (PTA) method (150 mg/L) compared with a uricase technique (40 mg/L). After ultrafiltration, the positive interference for the direct PTA method was retained in the protein fraction, but not in the filtrate. Adding exogenous etoposide to drug-free serum confir...

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The Photometric Determination of Cystine , Cysteine , Ascorbic Acid , and Related Com - Pounds with Phosphotungstic Acid

Some time ago a brief report (1) was made on the use of the Pulfrich photometer for the determination of sulfhydryl and disulfide compounds by a modified Folin (2) method. Since then the method has been extensively used in this laboratory (3-9). Folin’s method is based upon the observation that cystine plus sulfite gives a blue color with phospho-18-tungstic acid; whereas cystine alone develops...

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ژورنال

عنوان ژورنال: Journal of Histochemistry & Cytochemistry

سال: 1966

ISSN: 0022-1554,1551-5044

DOI: 10.1177/14.4.345